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Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana
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JoVE Journal Bioingegneria
Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

Optimizing the Use of a Liquid Handling Robot to Conduct a High Throughput Forward Chemical Genetics Screen of Arabidopsis thaliana

DOI:
10.3791/57393-v

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11:58 min

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April 30, 2018

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, ,
1Department of Horticulture,University of Kentucky

Capitoli

  • 00:05Titolo
  • 00:56Creating a Dilution Library
  • 05:03Adding Media-seed Mixture to Screening Plates
  • 06:40Adding Small Molecules to Screening Plates
  • 10:16Results: Representative Results from a Forward Chemical Genetics Screen on Arabidopsis
  • 11:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

A high throughput screen of synthetic small molecules was conducted on the model plant species, Arabidopsis thaliana. This protocol, developed for a liquid handling robot, increases the speed of forward chemical genetics screens, accelerating the discovery of novel small molecules affecting plant physiology.

Tags

Keywords: Liquid Handling RobotHigh ThroughputForward Chemical Genetics ScreenArabidopsis ThalianaSmall Molecule ScreeningAutomationDilution Library96-well PlatesPipette TipsLiquid HandlingDilutionChemical Library

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