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正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma – An Improved Protocol
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正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议

DOI:
10.3791/57400-v

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10:54 min

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April 04, 2018

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, , , , , , , , ,
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg

Capitoli

  • 00:04Titolo
  • 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
  • 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
  • 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
  • 08:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

肿瘤内皮细胞是肿瘤微环境和病程的重要决定因素。本文介绍了一种从人大肠癌和正常结肠分离纯化和存活的内皮细胞的协议, 用于药物检测和病机研究。

Tags

Endothelial CellsColorectal CarcinomaTumor BiologyAngiogenesisCell Isolation ProtocolHBSS BufferDMEM MediumCell StrainerCentrifugation

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