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Isolering af menneskelige endotelceller fra normale kolon og Colorectal karcinom - en forbedret protokol
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1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
Capitoli
- 00:04Titolo
- 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
- 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
- 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
- 08:59Conclusion
Tumor endotelceller er vigtige determinanter af tumor mikromiljø og forløbet af sygdommen. Her, er en protokol til isolering af ren og levedygtige endotelceller fra menneskelige colorectal karcinom og normal kolon skal anvendes i drug test og patogenese forskning beskrevet.
