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Immunologia e infezioni

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人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

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人干扰素α超灵敏单分子阵列数字酶联免疫吸附试验的研制与验证

DOI:

10.3791/57421-v

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08:26 min

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June 14, 2018

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Alba Llibre*^1,2, Vincent Bondet*^1,2, Mathieu P. Rodero, David Hunt, Yanick J. Crow⁵, Darragh Duffy²

¹Immunobiology of Dendritic Cells,Institut Pasteur, ²INSERM U1223, ³Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163,Institut Imagine, ⁴MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine,University of Edinburgh, ⁵Manchester Centre for Genomic Medicine,University of Manchester

Capitoli

00:05Titolo
01:03Paramagnetic Bead Preparation, Activation, and Antibody-conjugation
03:26Bead Blocking and Final Clean-up
04:50Assay Optimization
07:06Results: Application of an Optimized IFN-α Assay
07:40Conclusion

Summary

Traduzione automatica

在这里, 我们提出一个协议来描述一个单一的分子阵列数字 ELISA 检测的发展和验证, 这使得对所有干扰素α亚型的超敏检测在人体标本。

Tags

Keywords: Single Molecule Array Digital ELISA Interferon-Î³ Ultrasensitive Biomarker Discovery Autoimmune Polyendocrine Syndrome Type 1 Paramagnetic Beads EDC Antibody Conjugation

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