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Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation
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Biologia
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JoVE Journal Biologia
Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation

DOI:
10.3791/57422-v

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08:52 min

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July 15, 2018

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, , ,
1Plant Molecular and Cellular Biology Program,University of Florida, 2Department of Biology,University of Florida, 3Genetics Institute (UFGI),University of Florida, 4Alkali Soil Natural Environmental Science Center,Northeast Forestry University, 5Interdisciplinary Center for Biotechnology Research (ICBR),University of Florida

Capitoli

  • 00:02Titolo
  • 01:19Preparation of Enriched Stomata
  • 03:14Protein Extraction and SDS-PAGE Separation
  • 06:14Results: Stomata Tape-Peel Allows for the Preparation of High Quality Enriched Stomata Suitable for Conducting Physiological, Biochemical and Molecular Assays
  • 07:38Conclusion

Summary

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Traduzione automatica

This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.

Tags

Keywords: StomataGuard CellsEnrichmentIsolationTranscriptomicsProteomicsMetabolomicsBrassicaceaeArabidopsisScotch TapeCell Wall DigestionPhenol ExtractionProtein Precipitation

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