Rivista
/
Bioingegneria
/
用 Widefield 高含量分析系统实现荧光标记组织的自动滑动扫描和分割
JoVE Journal
Bioingegneria
This content is Free Access.
JoVE Journal Bioingegneria
Automated Slide Scanning and Segmentation in Fluorescently-labeled Tissues Using a Widefield High-content Analysis System
Please note that all translations are automatically generated. Click here for the English version.

用 Widefield 高含量分析系统实现荧光标记组织的自动滑动扫描和分割

DOI:
10.3791/57440-v

•

09:33 min

•

May 03, 2018

•

, , , ,
1Department of Clinical Neurosciences, Arnie Charbonneau Cancer Institute,University of Calgary, 2Department of Cell Biology and Anatomy, Hotchkiss Brain Institute,University of Calgary, 3Department of Oncology, Hotchkiss Brain Institute,University of Calgary

Capitoli

  • 00:04Titolo
  • 00:51Preview Scan and High Magnification Setup
  • 02:51Preview Scan and High Magnification Image Acquisition
  • 07:54Results: Representative Images and Analyses
  • 09:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们描述了一个协议, 在幻灯片上使用 widefield 高含量分析系统 (WHCAS) 自动分割荧光标记的组织。该协议在任何领域都有广泛的应用, 包括生物科学、医学工程和健康科学等生物组织中荧光标记的定量。

Tags

Automated Slide ScanningWidefield High-content Analysis SystemProtein QuantificationFluorescent MicroscopyImage-based FocusingAutomated Segmentation Workflow

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/57440?article_type=v

刀口扫描显微镜的样品制备，图像和分析协议
check_url/it/57440?article_type=v

视频率扫描共聚焦显微镜和内窥镜
check_url/it/57440?article_type=v

的系统分析<em>体外</em>细胞滚动使用多孔板微流控系统
check_url/it/57440?article_type=v

订货单细胞和单胚胎的三维约束：高内涵筛选新设备
check_url/it/57440?article_type=v

三维全层皮肤的生成等效和自动伤人
check_url/it/57440?article_type=v

基于自动对准的大面积扫描探针光刻及其在细胞培养研究中的应用
check_url/it/57440?article_type=v

用 Widefield 荧光显微镜研究软硅基美国匹茨堡的刚度测量
check_url/it/57440?article_type=v

利用自动高通量微流控系统对铜绿假单胞菌剪切应力的活细胞分析
check_url/it/57440?article_type=v

使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体
check_url/it/57440?article_type=v

用于刺激生物组织的电动和磁场设备

Read Article