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足月胎盘胎膜中原发性人蜕细胞的分离
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Medicina
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JoVE Journal Medicina
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
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足月胎盘胎膜中原发性人蜕细胞的分离

DOI:
10.3791/57443-v

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07:37 min

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April 30, 2018

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, , ,
1Department of Physiology,University of Toronto, 2Lunenfeld-Tanenbaum Research Institute,Sinai Health System, 3Department of Obstetrics and Gynecology,University of Toronto

Capitoli

  • 00:05Titolo
  • 00:42Collection of Decidual Tissue from Term Placental Membranes
  • 01:57Washing and Enzymatic Digestion of the Decidual Tissue
  • 03:56Generation of Single Cell Suspension
  • 05:22Results: Use of Flow Cytometry and Immunocytochemistry to Isolate the Primary Human Decidual Cells
  • 07:11Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议展示了一种从足月胎盘的胎儿细胞膜中分离出的主要人类蜕细胞的方法, 可用于各种应用 (即免疫细胞化学、流式术中、等), 目的是研究不同细胞群在妊娠并发症中的作用。

Tags

Primary Decidual CellsHuman ReproductionBlastocyst ImplantationLabor InitiationPregnancy-related PathologiesCell Isolation ProtocolTerm PlacentaEnzymatic DigestionCell Viability

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