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1Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, 2Program in Cancer Biology,Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, 4Department of Biology,Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
Capitoli
- 00:04Titolo
- 01:02Plasmid Preparation and gRNA Insertion
- 03:26Lentivirus Production
- 05:12Lentivirus Transduction of Cells / Cell Dimerization
- 07:08Results: CLOuD9 Induces Reversible β-Globin Promoter-LCR Looping
- 08:55Conclusion
Chromatin looping spiller en viktig rolle i genreguleringen; imidlertid har det vært ingen teknologiske fremskritt som tillate selektiv og reversibel endring av chromatin looper. Her beskriver vi et kraftig system for chromatin loop reorganisering bruker CRISPR-dCas9 (CLOuD9), viste til selektivt og reversibel modulerer genuttrykk på målrettet loci.
