Rivista
/
Immunologia e infezioni
/
小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Please note that all translations are automatically generated. Click here for the English version.

小鼠骨髓源性巨噬细胞 Inflammasome 活化和 Pyroptotic 细胞死亡的检测

DOI:
10.3791/57463-v

•

06:52 min

•

May 21, 2018

•

,
1Department of Laboratory Medicine,University of Washington

Capitoli

  • 00:04Titolo
  • 00:45NOD-like RecptorP3 (NLRP3) Inflammasome Activation, Caspase-1 Activity Probe (FAM-YVAD-FMK)-labeling, and Antibody Staining
  • 04:07Lactate Dehydrogenase (LDH) Release Assay
  • 05:23Results: Representative Inflammasome Activation and Pyroptotic Cell Death Detection
  • 06:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

我们描述了 NLRP3 inflammasome 激活的细胞基础上的荧光显微镜和染色的主动 caspase-1 和适配器, ASC。以乳酸脱氢酶释放试验为依据, 检测 pyroptotic 裂解的人群。这些技术可以适应研究 inflammasome 生物学的许多方面。

Tags

Keywords: Inflammasome ActivationPyroptosisMacrophagesFluorescence MicroscopyLDH Release AssayCaspase-1NigericinFAM-YVAD-FMKParaformaldehydeConfocal Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
check_url/it/57463?article_type=v

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签
check_url/it/57463?article_type=v

使用骨髓源性巨噬细胞巨噬细胞极化的调查

骨髓衍生的巨噬细胞产
check_url/it/57463?article_type=v

激活和NLRP3炎性活性的人单核细胞来源的树突状细胞用IL-1β测量
check_url/it/57463?article_type=v

隔离和小鼠骨髓的静脉注射单核细胞衍生
check_url/it/57463?article_type=v

偏光M1和M2骨髓来源的巨噬细胞的使用实时外流量分析代谢表征
check_url/it/57463?article_type=v

股骨窗口商会模型<em>在体内</em在小鼠骨髓>手机跟踪
check_url/it/57463?article_type=v

分离及小鼠淋巴细胞的激活
check_url/it/57463?article_type=v

抗体药物对小鼠骨髓及腹腔巨噬细胞活化作用的评价

Read Article