/

/

MALDI 质谱法测定小鼠组织提取物蛋白酶特异性: 操作 PH 值导致特异性改变

JoVE Journal
Biochimica

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biochimica

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Please note that all translations are automatically generated. Click here for the English version.

MALDI 质谱法测定小鼠组织提取物蛋白酶特异性: 操作 PH 值导致特异性改变

DOI:

10.3791/57469-v

•

09:47 min

•

May 25, 2018

•

Hiroyuki Yamamoto, Yoshikazu Sawaguchi, Michio Kimura

¹Department of Microbiology and Molecular Cell Biology,Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics,Nihon Pharmaceutical University

Capitoli

00:05Titolo
00:36Preparation of Tissue Extracts
01:40Digestion of Substrates in Model Protease and Tissue Extracts
02:59Purification of the Iminobiotin-labeled Substrate
05:17Sample Preparation and Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF Mass Spectrometry)
06:48Results: Identification of Protease Specificity
08:46Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 以确定蛋白酶特异性的粗鼠组织提取物使用 MALDI 的光谱。

Tags

Protease Specificity MALDI-TOF Mass Spectrometry PH Manipulation Protease Assay Biotin Labeling Streptavidin Beads

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

种测定和定量混合物中使用肽应用的MRM质谱肉认证

时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态

解决由蓝变性PAGE和蛋白质相关剖析亲和纯化的蛋白复合物

用肽微阵列分析组蛋白抗体特异性

用氢-氘交换质谱法定义 Hsp33's 氧化还原调节陪护活动和 Hsp33 映射构象变化

单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法

通过肽浓缩和质谱检测蛋白质泛化位点检测

使用开源 MALDI TOF-MS IDBac 管道分析微生物蛋白和专用代谢物数据

血管紧张蛋白II图解的硝酸分析与鉴定

使用液色谱结合串联质谱法对糖皮质酶细胞脂质的定量分析

Read Article