CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Benjamin L. Kidder

doi:10.3791/57481

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CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

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Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

DOI:

10.3791/57481-v

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11:13 min

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May 25, 2018

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Benjamin L. Kidder²

¹Department of Oncology,Wayne State University School of Medicine, ²Karmanos Cancer Institute,Wayne State University School of Medicine

Capitoli

00:04Titolo
01:02Crosslinking of Embryonic Stem Cells for ChIP and CARIP
02:29Chromatin Sonication for ChIP and CARIP
03:48ChIP and CARIP Process
07:12Double-stranded cDNA Synthesis for CARIP-Seq
08:53Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
10:43Conclusion

Summary

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Here, we describe methods to perform ChIP-Seq and CARIP-Seq, including library preparation for next-generation sequencing, to generate global epigenomic and chromatin-associated RNA maps in ES cells.

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

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