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CARIP-Seq ו שבב-Seq: שיטות לזיהוי RNAs הקשורים כרומטין והן אינטראקציות חלבון-DNA בתאי גזע עובריים
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Capitoli
- 00:04Titolo
- 01:02Crosslinking of Embryonic Stem Cells for ChIP and CARIP
- 02:29Chromatin Sonication for ChIP and CARIP
- 03:48ChIP and CARIP Process
- 07:12Double-stranded cDNA Synthesis for CARIP-Seq
- 08:53Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
- 10:43Conclusion
Tags
CARIP-SeqChIP-SeqChromatin-associated RNAsProtein-DNA InteractionsEmbryonic Stem CellsEpigeneticsTranscription FactorsEpigenetic RegulatorsHistone ModificationsChromatin ImmunoprecipitationSonicationFormaldehyde Cross-linkingGlycinePhosphate Buffered SalineTrypsinizationMEF MediumFetal Bovine SerumSonication BufferSodium Dodecyl Sulfate
