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A 3D Organotypic Melanoma Spheroid Skin Model
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
A 3D Organotypic Melanoma Spheroid Skin Model

A 3D Organotypic Melanoma Spheroid Skin Model

DOI:
10.3791/57500-v

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08:49 min

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May 18, 2018

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,
1Experimental Dermatology, Medical Faculty,TU-Dresden

Capitoli

  • 00:04Titolo
  • 01:20Generation of 3D Melanoma Spheroids via the Hanging Drop Method
  • 03:16Generation of the Dermal and Epidermal Counterparts of Organotypic Full Skin Reconstructs
  • 05:08Air-liquid Cultivation of Organotypic Full Skin Reconstructs and Generation of Organotypic Melanoma Spheroid Skin Reconstructs
  • 06:45Results: Validation of Organotypic Full Skin Reconstruct and Melanoma Spheroid Skin Reconstruct
  • 08:06Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a protocol to generate a 3D organotypic melanoma spheroid skin model that recapitulates both the architecture and multicellular complexity of an organ/tumor in vivo but at the same time accommodates systematic experimental intervention.

Tags

3D Organotypic Melanoma Spheroid Skin ModelTumor-host InteractionTherapeutic Intervention3D ArchitectureNon-vascularized MetastasesTumor HeterogeneityPrimary CellsMelanoma Cells 451LUMelanoma SpheroidsHanging DropSkin ReconstructsDermal Counterpart

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