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利用相扑融合策略生成国产、未加标签的杭丁顿蛋白 Exon1 单体和纤维
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
DOI:

11:22 min

June 27, 2018

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Capitoli

  • 00:04Titolo
  • 00:53Cell Lysis and Purification of His6-SUMO Httex1 Fusion Protein by Immobilized Metal Affinity Chromatography (IMAC)
  • 02:56Cleavage of the His6-SUMO-tag and HPLC Purification
  • 06:06Disaggregation and Resolubilization of Httex1 Proteins
  • 07:53Results: Characterization of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Prepared via SUMO Fusion
  • 09:52Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个健壮和优化的协议, 以生产毫克数量的本地, 无标签单体和纤维的 exon1 的杭丁顿蛋白蛋白 (Httex1) 的基础上的瞬态融合的小泛素相关修饰剂 (相扑)。

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