/

Immunologia e infezioni

/

成年日本血吸虫细胞外囊泡的分离与鉴定

JoVE Journal
Immunologia e infezioni

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Immunologia e infezioni

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Please note that all translations are automatically generated. Click here for the English version.

成年日本血吸虫细胞外囊泡的分离与鉴定

DOI:

10.3791/57514-v

•

07:37 min

•

May 22, 2018

•

Juntao Liu*¹, Lihui Zhu*¹, Lihui Wang², Yongjun Chen, Bikash Ranjan Giri, Jianjun Li, Guofeng Cheng

¹Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, ²Tianjin Agricultural University

Capitoli

00:04Titolo
00:46Isolating SjEVs from Schistosoma japonicum Cultures
03:00Characterization of EVs by Western Blotting and TEM
04:01Labeling Schistosoma japonicum Extracellular Vesicles with PKH67 Followed by Cell Uptake
06:15Results: Schistosoma japonicum Extracellular Vesicle Characterization
07:17Conclusion

Summary

Traduzione automatica

在这里, 我们提出一个协议, 以描述细胞外囊泡 (电动车) 隔离在体外培养培养基从成年日本血吸虫。

Tags

Extracellular Vesicles Schistosoma Japonicum In Vitro Culture Isolation Purification Centrifugation Dialysis PEG8000 Western Blot

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Cercarial转型和在体外栽培曼氏血吸虫 Schistosomules

从使用鸡蛋<em>血吸虫</em>作为<em>在体内</em>蠕虫引起的肺部炎症模型

从老鼠肠道树突状细胞和巨噬细胞的分离和表征

简化人力中性粒细胞胞外陷阱（英语教师）分离和处理

隔离和中性粒细胞的表征与抗肿瘤性质

分离，鉴定和牙龈免疫细胞网络的功能性考试

小鼠小肠淋巴细胞的分离和流式细胞表征

流量Virometry来分析病毒颗粒的抗原谱和外囊泡

受肥胖相关炎症组织的巨噬细胞的分离、鉴定和纯化

中性粒细胞衍生微粒的分离与鉴定功能研究

Read Article