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A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
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JoVE Journal Biologia dello sviluppo
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes

DOI:
10.3791/57556-v

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10:56 min

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September 15, 2018

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, , , , , , , , , , , , , , ,
1Department of Medicine,University of Hong Kong-Shenzhen Hospital, 2The Cardiology Division, Department of Medicine, Li Ka Shing Faculty of Medicine,University of Hong Kong, 3School of Biomedical Sciences, Institute of Vascular Medicine, Li Ka Shing Institute of Health Sciences,Chinese University of Hong Kong, 4Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences, Joint School of Life Sciences,Guangzhou Institutes of Biomedicine and Health and Guangzhou Medical University, 5Laboratory of RNA, Chromatin, and Human Disease, CAS Key Laboratory of Regenerative Biology and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences, 6Research Centre of Heart, Brain, Hormone, and Healthy Ageing, Li Ka Shing Faculty of Medicine,University of Hong Kong, 7Hong Kong-Guangdong Stem Cell and Regenerative Medicine Research Centre,University of Hong Kong and Guangzhou Institutes of Biomedicine and Health

Capitoli

  • 00:04Titolo
  • 00:53Dissociation of iHeps for Injection
  • 04:22Intrasplenic Injection of iHeps
  • 06:11Endothelial Function Test
  • 09:18Results: iHep Mediated Repopulation of LRG Mice Liver
  • 10:39Conclusion

Summary

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Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.

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Keywords: Familial HypercholesterolemiaHuman Liver Chimeric Mouse ModelInduced Pluripotent Stem Cell-derived HepatocytesIn Vivo Drug TestingInherited Liver DiseasesCell DissociationSingle-cell SuspensionCell Strainer

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