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Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
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JoVE Journal Biochimica
Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

DOI:
10.3791/57565-v

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10:27 min

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May 04, 2018

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, ,
1Department of Biology,Saint Louis University

Capitoli

  • 00:04Titolo
  • 00:39WST-1 Reduction Assay
  • 04:23DPIP Reduction Assay
  • 06:17Determination of Whether Reduced Electron Acceptors are Substrates for AO or SOD
  • 07:59Results: Measuring Trans-Plasma Membrane Electron Transport
  • 09:34Conclusion

Summary

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The goal of this protocol is to spectrophotometrically monitor trans-plasma membrane electron transport utilizing extracellular electron acceptors and to analyze enzymatic interactions that may occur with these extracellular electron acceptors.

Tags

Keywords: C2C12 MyotubesTrans-plasma Membrane Electron TransportSpectrophotometric AssayWST-1PMSGlucosePBSReal-time MonitoringRedox BiologyCell CultureDifferentiationAbsorbance Measurement

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