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小鼠巨噬细胞吞噬的延时3D 成像

JoVE Journal
Immunologia e infezioni

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JoVE Journal Immunologia e infezioni

Time-lapse 3D Imaging of Phagocytosis by Mouse Macrophages

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小鼠巨噬细胞吞噬的延时3D 成像

DOI:

10.3791/57566-v

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07:24 min

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October 19, 2018

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Markus Horsthemke, Janine Wilden, Anne C. Bachg, Peter J. Hanley

¹Institut für Molekulare Zellbiologie

Capitoli

00:04Titolo
01:00Resident Mouse Peritoneal Macrophage and Human Red Blood Cell Isolation
04:00Macrophage Plasma Membrane Labeling and Human Red Blood Cell-mouse Immunoglobulin (IgG) Opsonization and Imaging
06:32Results: Representative Time-lapse, 3D-imaging of Phagocytosis
07:02Conclusion

Summary

Traduzione automatica

本文介绍了利用旋转圆盘共聚焦显微镜对小鼠腹腔巨噬细胞单个吞噬事件进行图像的方法。这些协议可以扩展到其他吞噬细胞。

Tags

Phagocytosis Macrophages Time-lapse 3D Imaging Cell Isolation Peritoneal Lavage RPMI Medium HEPES Fibronectin-coated Slides Air Bubble Removal Cell Incubation Microscopy

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