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背根神经节分离与原代培养对神经递质释放的研究

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

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背根神经节分离与原代培养对神经递质释放的研究

DOI:

10.3791/57569-v

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08:15 min

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October 06, 2018

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Ya-Tin Lin, Jin-Chung Chen^2,3

¹Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology,Chang Gung University, ²Healthy Aging Research Center,Chang Gung University, ³Neuroscience Research Center,Chang Gung Memorial Hospital

Capitoli

00:04Titolo
00:32Isolation of Rat Lumbar DRG
01:40Primary Culture of Rat DRG
04:22Transfection of DRG Cells with NPFFR2 siRNA
05:44Release of Neurotransmitters from Primary DRG Cells
06:42Results: Stimulation-induced Release of Neurotransmitters from Cultured DRG Cells
07:36Conclusion

Summary

Traduzione automatica

背根神经节的原代培养常用于研究感官神经元的生理功能或病理相关事件。在这里, 我们展示了使用腰椎背根神经节的文化, 以检测神经递质的释放后神经元肽 FF 受体2型刺激与选择性激动剂。

Tags

Dorsal Root Ganglia Primary Culture Neurotransmitter Release Sensory Neurons Nociception Rat Spinal Cord Lumbar DRG Collagenase Trypsin Centrifugation Cell Dissociation

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