È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Shanghai Key Clinical Disciplines of otorhinolaryngology,Fudan University, 2Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Department of Medical Microbiology and Parasitology, School of Basic Medical Science,Shanghai Medical College of Fudan University, 3Department of Dermatology, Huashan Hospital,Fudan University
Capitoli
- 00:04Titolo
- 00:40Biofilm Formation and Light Irradiation
- 02:17Photodynamic Therapy Analysis
- 04:00Results: Representative Biofilm Viability Analyses
- 04:33Conclusion
This manuscript describes a protocol to study the antimicrobial effect of 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT) on a Staphylococcus aureus biofilm. This protocol can be used to develop an in vitro model to study the treatment of bacterial biofilms with PDT in the future.
