È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Titolo
- 00:44Germinating Potato in Sterilized Conditions
- 01:56Collecting Potato Root Exudates
- 03:23Treatment and Sampling of Bacteria
- 04:57RNA Isolation
- 07:12Results: RNA Quality Check and Ratio Intensity Plot for Visualizing Differential Gene Expression
- 08:30Conclusion
The goal of the protocol presented here is to study the transcriptomic response of endosphere-isolated Bacillus mycoides to potato root exudates. This method facilitates the identification of important bacterial genes involved in plant-microbe interactions and is in principle applicable to other endophytes and plants, with minor adjustments.
