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Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach

DOI:
10.3791/57610-v

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08:24 min

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May 02, 2018

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, , , ,
1Department of Biomedicine,University of Basel, 2Division of Gastroenterology and Hepatology,University Hospital of Basel

Capitoli

  • 00:05Titolo
  • 00:57Intraperitoneal Injection of LPS to Mice
  • 01:53Tissue Sampling for Ribonucleic Acid (RNA) Extraction and Homogenization
  • 03:22RNA Extraction from Tissue
  • 05:47Digestion of Remaining DNA
  • 06:51Results: LPS Injection Induces the Expression of Pro-inflammatory Cytokines in C57Bl/6 Mice
  • 07:43Conclusion

Summary

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Here a protocol to mimic the entrance of bacterial-derived compounds after intestinal barrier breach is presented. A low sublethal dose of lipopolysaccharide was injected systemically into mice, which were monitored for 24 h post-injection. The expression of pro-inflammatory cytokines was determined at several time points in spleen, liver, and colon.

Tags

LipopolysaccharideIntestinal Barrier BreachMicrobial-derived ProductsImmune ResponseInflammatory Bowel DiseasePeritoneal Cavity InjectionEndotoxemiaRNA IsolationOrgan Homogenization

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