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膜片钳后单细胞多重反转录聚合酶链反应

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

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膜片钳后单细胞多重反转录聚合酶链反应

DOI:

10.3791/57627-v

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10:44 min

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June 20, 2018

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Gabrielle Devienne*¹, Benjamin Le Gac*¹, Juliette Piquet*¹, Bruno Cauli

¹UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine – Institut de Biologie Paris Seine (NPS – IBPS),Sorbonne Université

Capitoli

00:04Titolo
00:57Acute Slice Preparation
02:53Single Cell RT-PCR after Patch-clamp Recording
05:54PCR Validation
08:47Results: Characterization of Layer V Neocortical Pyramidal Cells
09:57Conclusion

Summary

Traduzione automatica

本协议描述了在膜片钳后执行单细胞多重反向转录聚合酶链反应所需的关键步骤和预防措施。该技术是一种简单有效的方法, 用于分析由膜片钳记录的单个细胞所确定的一组基因的表达谱。

Tags

Keywords: Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction Patch-clamp Neuroscienze Neuronal Population Transmission Systems Gene Expression Astrocytes Animal Models Brain Dissection Vibratome Sectioning Whole-cell Recording Cytoplasm Harvesting RT-PCR GDNA Contamination

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