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Visualisering af Amyloid β indskud i den menneskelige hjerne med Matrix assisted Laser Desorption/Ionisation Imaging massespektrometri
JoVE Journal
Neuroscienze
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JoVE Journal Neuroscienze
Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Visualisering af Amyloid β indskud i den menneskelige hjerne med Matrix assisted Laser Desorption/Ionisation Imaging massespektrometri

DOI:
10.3791/57645-v

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09:31 min

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March 07, 2019

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, , , , , ,
1Department of Life and Medical Systems,Doshisha University, 2Bruker Daltonics K.K., 3The Brain Bank for Aging Research,Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 4Graduate School of Brain Science,Doshisha University

Capitoli

  • 00:04Titolo
  • 00:51Preparation of Tissue Sections for Imaging Mass Spectrometry (IMS)
  • 05:05MALDI-IMS
  • 06:12Results: Visualization of Aβ and Its Isoforms from Autopsied Brains with AD and CAA with MALDI-IMS
  • 08:14Conclusion

Summary

Traduzione automatica

Traduzione automatica

Molekylær billeddannelse med matrix assisted laser desorption/Ionisation-baseret tænkelig massespektrometri (MALDI-IMS) tillader den samtidige kortlægning af flere analysander i biologiske prøver. Vi præsenterer her, en protokol til påvisning og visualisering af amyloid β protein på hjernen væv af Alzheimers sygdom og cerebral amyloid angiopathie prøver ved hjælp af MALDI-IMS.

Tags

Keywords: Amyloid BetaMALDI Imaging Mass SpectrometryAlzheimer’s DiseaseAutopsyFormic AcidSegmentation MapsMarker ProteinsPeptidesPlaqueSubarachnoid VasculatureCryostatITO-coated Glass SlidesEthanol WashVacuum Drying

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