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Journal / Biochemistry / 小さい gtp アーゼ プレニル化および分子量 GTP 結合を使用して膜分画と gtp…
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Biochemistry
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JoVE Journal Biochemistry
小さい gtp アーゼ プレニル化および分子量 GTP 結合を使用して膜分画と gtp アーゼ抗体法の検出
 
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小さい gtp アーゼ プレニル化および分子量 GTP 結合を使用して膜分画と gtp アーゼ抗体法の検出

Article DOI: 10.3791/57646-v • 13:52 min • November 11th, 2018
November 11th, 2018 •
1,2, 1,2,3, 1, 1,2, 4, 5, 6,7,8, 1,9, 1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences

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ここでプレニル化とグアノシン 5'-三リン酸 (GTP) を調査するためのプロトコルを説明-GTPase の読み込み。このプロトコルは、2 つの詳細なメソッドで構成されています、すなわち膜分画と gtp アーゼ抗体アッセイします。プレニル化と GTP を測定するプロトコルを使用することができます別の他の低分子量 g タンパク質の読み込み。

Tags

生化学、問題 141、シンバスタチン、膠芽腫、Rho GTPase、がん細胞の生物学、プレニル化、メバロン酸経路
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