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Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
JoVE Journal
Medicina
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JoVE Journal Medicina
Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

DOI:
10.3791/57647-v

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08:55 min

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May 18, 2018

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, , ,
1Department of Clinical Sciences,North Carolina State University

Capitoli

  • 00:04Titolo
  • 00:44Ischemia and Tissue Collection
  • 02:54Crypt Stem Cell Isolation
  • 06:12Results: Representative Crypt Isolation and Enteroid Generation
  • 07:48Conclusion

Summary

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This protocol will enable readers to successfully establish a porcine model of segmental intestinal ischemia and subsequently isolate and culture intestinal stem cells for the study of epithelial repair following injury.

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Keywords: Intestinal Stem CellsIschemiaPorcine ModelSmall IntestineCrypt IsolationEpithelial Stem CellsDissociation

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