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Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
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Biologia
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JoVE Journal Biologia
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

DOI:
10.3791/57649-v

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06:38 min

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May 03, 2018

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, , ,
1Department of Molecular, Cell, and Developmental Biology,University of California, Santa Cruz

Capitoli

  • 00:05Titolo
  • 01:06Urine Depletion and Bladder Rinsing
  • 02:40Plasmid Delivery
  • 05:16Results: Successful Delivery of pCAG-GFP Plasmid to the Mouse Bladder Urothelium
  • 06:13Conclusion

Summary

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We describe a novel method for the delivery of DNA plasmid into the urothelial cells of mouse bladder in vivo through urethra catheterization and electroporation. It offers a fast and convenient way for generating autochthonous mouse models of bladder diseases.

Tags

Plasmid DNA DeliveryBladder UrotheliumElectroporationGene OverexpressionGenome EditingBladder CancerGene TherapyAnesthesiaCatheterPBS WashingDNA PlasmidTrypan BlueVertical Incision

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