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Lys-ark mikroskopi for tre-dimensionelle visualisering af Human immun celler
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Capitoli
- 00:04Titolo
- 00:53Preparation of Neutralized Collagen
- 01:55Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
- 04:08Image Acquisition Using Light-sheet Microscopy
- 05:07Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
- 07:17Results: Visualization of Human CTL Cells by Light-sheet Microscopy
- 08:32Conclusion
Vi præsenterer her, en protokol for at visualisere immunceller indlejret i en tre-dimensionelle (3D) kollagen matrix ved hjælp af lys-ark mikroskopi. Denne protokol også uddyber hvordan man spore celle migration i 3D. Denne protokol kan være ansat af andre former for affjedring celler i den 3D matrix.
