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基质辅助激光解吸/电离质谱中增强碳水化合物离子信号的高效样品制备方法

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Chimica

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JoVE Journal Chimica

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

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基质辅助激光解吸/电离质谱中增强碳水化合物离子信号的高效样品制备方法

DOI:

10.3791/57660-v

•

07:12 min

•

July 29, 2018

•

Yu-Meng Ou², Shu-Yun Kuo, Hsun Lee, Huan-Tsung Chang, Yi-Sheng Wang

¹Genomic Research Center,Academia Sinica, ²Department of Chemistry,National Taiwan University

Capitoli

00:04Titolo
00:45Cleaning Sample Plate
01:41Sample Deposition and Recrystallization (Small Amount of Sample)
03:20Analysis of a 1 μL Sample
04:22Mass Spectrometry Data Acquisition and Analysis
05:04Results: Carbohydrate Ion Signals in Recrystallized Structures by MALDI Mass Spectrometry
06:34Conclusion

Summary

Traduzione automatica

本文演示了在样品制备过程中, 通过改造晶体结构来增强 MALDI 质谱中的碳水化合物离子信号的协议。

Tags

Keywords: Matrix-assisted Laser Desorption/ionization Mass Spectrometry Sample Preparation Carbohydrate Ion Signals Methanol Depressurization 2 5-dihydroxybenzoic Acid Sialyl-lewis A Maltoheptaose Crystal Morphology

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