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应用 Fraumeni 综合征诱导多潜能干细胞建模骨肉瘤
JoVE Journal
Ricerca sul cancro
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JoVE Journal Ricerca sul cancro
Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells
DOI:

08:52 min

June 13, 2018

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Capitoli

  • 00:04Titolo
  • 00:47Differentiation of Li-Fraumeni Syndrome (LFS) Patient-derived Induced Pluripotent Stem Cell (iPSC) to Mesenchymal Stem Cell (MSC) (Days 0 – 28)
  • 02:19iPSC-derived MSC Maturation (Day 28 – 45) and Differentiation of LFS MSC to Osteoblasts (Day 0 – 24)
  • 04:33Xenograft In Vivo Tumorigenesis Model Setup
  • 06:39Results: Representative LFS MSC Differentiation
  • 08:10Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个由李-Fraumeni 综合征 (LFS) 患者衍生成纤维细胞生成诱导多能干细胞 (iPSCs) 的协议, 通过间充质干细胞 (MSCs) 分化成成骨细胞, 并在体内建模利用 LFS 患者源性成骨细胞进行肿瘤发生。

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