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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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JoVE Journal Ricerca sul cancro
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

DOI:
10.3791/57685-v

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08:32 min

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June 07, 2018

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, ,
1Department of Otolaryngology-Head and Neck Surgery,Baylor College of Medicine, 2Interdepartmental Graduate Program in Translational Biology and Molecular Medicine,Baylor College of Medicine

Capitoli

  • 00:04Titolo
  • 00:55Tumor Harvest and Digestion
  • 03:02Immune and Tumor Cellular Fraction Separation and Labeling
  • 05:57Results: Representative Tumor Component Features
  • 07:27Conclusion

Summary

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Here we describe a method to separate and enrich components of the tumor immune and non-immune microenvironment in established subcutaneous tumors. This technique allows for the separate analysis of tumor immune infiltrate and non-immune tumor fractions which can permit comprehensive characterization of the tumor immune microenvironment.

Tags

Tumor Immune MicroenvironmentTumor Non-immune MicroenvironmentImmune OncologyTumor ProfilingTumor DissociationTumor EnrichmentTumor Cell FractionationTumor Immune Cell SeparationCollagenaseDNaseDensity Gradient MediumSubcutaneous Tumors

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