/

Ricerca sul cancro

/

肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定

JoVE Journal
Ricerca sul cancro

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Ricerca sul cancro

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Please note that all translations are automatically generated. Click here for the English version.

肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定

DOI:

10.3791/57685-v

•

08:32 min

•

June 07, 2018

•

Jared M. Newton*^1,2, Aurelie Hanoteau*¹, Andrew G. Sikora

¹Department of Otolaryngology-Head and Neck Surgery,Baylor College of Medicine, ²Interdepartmental Graduate Program in Translational Biology and Molecular Medicine,Baylor College of Medicine

Capitoli

00:04Titolo
00:55Tumor Harvest and Digestion
03:02Immune and Tumor Cellular Fraction Separation and Labeling
05:57Results: Representative Tumor Component Features
07:27Conclusion

Summary

Traduzione automatica

在这里, 我们描述了一个方法, 以分离和丰富的组成部分, 肿瘤免疫和非免疫微环境的建立皮下肿瘤。该技术允许对肿瘤免疫浸润和非免疫肿瘤分数进行单独分析, 从而可以对肿瘤免疫微环境进行综合表征。

Tags

Tumor Immune Microenvironment Tumor Non-immune Microenvironment Immune Oncology Tumor Profiling Tumor Dissociation Tumor Enrichment Tumor Cell Fractionation Tumor Immune Cell Separation Collagenase DNase Density Gradient Medium Subcutaneous Tumors

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

该<em>果蝇</em>成虫盘瘤模型：基因的表达与肿瘤侵袭性的可视化和定量遗传马赛克

小鼠原位膀胱肿瘤模型和肿瘤检测系统

Ex 活体人肺肿瘤切片中居民 CD8 T 淋巴细胞的共聚焦显微成像

将斑马鱼小儿脑肿瘤移植入免疫能力的宿主，用于长期研究肿瘤细胞行为和药物反应

单细胞下游特征的靶细胞预富集与全基因组扩增

一种确定环境富集对小鼠结肠肿瘤模型结肠菌群生物多样性影响的方法

缺血再灌注损伤中转移性肝肿瘤的模样

小肠神经内分泌肿瘤类球体的建立与特征

正交胰腺肿瘤的产生与肿瘤渗透T细胞细胞毒性的外体特征

通过实体瘤移植植入骨模拟原发性骨肿瘤和骨转移

Read Article