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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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Capitoli
- 00:04Titolo
- 01:35Fabrication of a PDMS Microfluidic Platform
- 03:16Production of Polyacrylamide Oligo-Microspheres
- 06:06Performing DNA Hybridization on the Surface of Polyacrylamide Oligomicrosphere
- 07:06Microsphere-PCR for Amplifying ssDNA
- 08:08Confocal Microscopy Acquisition
- 08:38“Results: Fabrication of Droplet-base Microfluidic Platform and Application of Microspheres for Microsphere-PCR Amplification”
- 10:33Conclusion
This work provides a method for the fabrication of droplet-based microfluidic platforms and the application of polyacrylamide microspheres for microsphere-PCR amplification. The microsphere-PCR method makes it possible to obtain single-stranded DNA amplicons without separating double-stranded DNA.
