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Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry
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JoVE Journal Bioingegneria
Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

Making Conjugation-induced Fluorescent PEGylated Virus-like Particles by Dibromomaleimide-disulfide Chemistry

DOI:
10.3791/57712-v

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10:18 min

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May 27, 2018

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, , ,
1Department of Chemistry & Biochemistry,University of Texas at Dallas, 2Undergraduate Biology,University of Texas at Dallas, 3Undergraduate Healthcare Studies,University of Texas at Dallas, 4Departments of Chemistry & Biochemistry and Biomedical Engineering,University of Texas at Dallas

Capitoli

  • 00:05Titolo
  • 00:41Expression of Qβ
  • 02:51Purification of Qβ
  • 05:51Conjugating DB Compounds on Qβ
  • 08:06Results: Qβ-PEG Can Be Used as a Fluorescence Probe Inside Cells
  • 09:35Conclusion

Summary

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Traduzione automatica

Here, we present a procedure to fluorescently functionalize the disulfides on Qβ VLP with dibromomaleimide. We describe Qβ expression and purification, the synthesis of dibromomaleimide-functionalized molecules, and the conjugation reaction between dibromomaleimide and Qβ. The resulting yellow fluorescent conjugated particle can be used as a fluorescence probe inside cells.

Tags

PEGylated Virus-like ParticlesDibromomaleimide-disulfide ChemistryBio-conjugationFluorescent FunctionalizationQBeta BacteriophageE. Coli BL21 ExpressionIPTG InductionCell LysisProtein Purification

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