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Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila
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Capitoli
- 00:04Titolo
- 00:45Tissue Preparation and Developmental Staging of Drosophila melanogaster Pupa
- 02:24Dissection of D. melanogasterAntennal Discs at 0 – 8 h APF
- 04:17Dissection of D. melanogaster Mid-pupal Antennae
- 05:43Dissection of D. melanogaster Adult Antennae
- 07:02Results: Analyses of Prepared Tissues
- 08:02Conclusion
Here, we present a protocol to stage and dissect developing olfactory tissue from Drosophila species. The dissected tissue can later be used for molecular analyses, such as quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) or RNA sequencing (RNAseq), as well as in vivo analyses such as immunohistochemistry or in situ hybridization.
