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小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
DOI:

09:40 min

June 20, 2018

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Capitoli

  • 00:04Titolo
  • 00:50Preparing Collagen I Plates
  • 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
  • 02:36Tissue Processing and Primary Tubular Cells Isolation
  • 04:11Mitochondria Bioenergetics Assay
  • 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
  • 09:04Conclusion

Summary

Traduzione automatica

该协议提供了一种经济高效的方法, 以分离和表征小鼠原发性肾管细胞, 随后可以进行亚培养, 以评估肾脏生物学功能, 包括线粒体生物能学。

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