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Isolation, karakterisering og High Throughput ekstracellulære Flux analyse af musen primær Renal tubulær epitelceller
JoVE Journal
Biologia
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JoVE Journal Biologia
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Isolation, karakterisering og High Throughput ekstracellulære Flux analyse af musen primær Renal tubulær epitelceller

DOI:
10.3791/57718-v

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09:40 min

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June 20, 2018

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, ,
1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine

Capitoli

  • 00:04Titolo
  • 00:50Preparing Collagen I Plates
  • 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
  • 02:36Tissue Processing and Primary Tubular Cells Isolation
  • 04:11Mitochondria Bioenergetics Assay
  • 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
  • 09:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne protokol giver en omkostningseffektiv tilgang for at isolere og karakterisere mus primær renal tubulær celler, der kan efterfølgende sub kulturperler for at vurdere renal biologiske funktioner ex vivo, herunder mitokondrie bioenergetik.

Tags

Keywords: Renal MetabolismFatty Acid OxidationRenal FibrosisNephropathiesMitochondrial BioenergeticsRenal Epithelial CellsHigh-throughput ScreeningRenal Metabolic DiseasePrimary Renal Tubular Epithelial CellsCollagen-coated DishesMouse Kidney PerfusionKidney DigestionTubular Cell Isolation

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