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Genetics

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JoVE Journal Genetics
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
 

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Article DOI: 10.3791/57737-v • 08:27 min • May 21st, 2018
May 21st, 2018 •
1,2, 1, 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

Capitoli

Riepilogo
Automatic Translation

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.

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Keywords: Bronchoalveolar Lavage Exosomes Lipopolysaccharide Septic Lung Injury Acute Lung Injury Acute Respiratory Distress Syndrome Alveolar Microenvironment Exosome Isolation Centrifugation Ultracentrifugation Syringe Filtration Exosome Characterization
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