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支气管肺泡灌洗外来体在脂多糖致脓毒性肺损伤中的作用
JoVE Journal
Genetica
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JoVE Journal Genetica
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
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支气管肺泡灌洗外来体在脂多糖致脓毒性肺损伤中的作用

DOI:
10.3791/57737-v

•

08:27 min

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May 21, 2018

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, ,
1Department of Veterans Affairs,Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine,Emory University

Capitoli

  • 00:04Titolo
  • 01:01Mouse Acute Septic Lung Injury
  • 02:05Exosome Isolation and Purification
  • 03:41Exosome Recipient Uptake and Interaction Validation
  • 05:30Results: BALF-isolated Exosome Characterization
  • 06:53Conclusion

Summary

Traduzione automatica

Traduzione automatica

暴露于腹腔内的 LPS 分泌外来体的小鼠支气管肺泡灌洗 (球) 液, 包装与 miRNAs。使用共培养系统, 我们表明, 外来体释放的球液扰乱支气管上皮细胞紧密连接蛋白的表达, 并增加支持炎症细胞因子的表达, 加重肺损伤。

Tags

Bronchoalveolar LavageExosomesLipopolysaccharideSeptic Lung InjuryAcute Lung InjuryAcute Respiratory Distress SyndromeAlveolar MicroenvironmentExosome IsolationCentrifugationUltracentrifugationSyringe FiltrationExosome Characterization

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