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Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

Subcellular Fractionation from Fresh and Frozen Gastrointestinal Specimens

DOI:
10.3791/57740-v

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10:07 min

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July 15, 2018

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, , , ,
1Department of Genetics, Physical Anthropology and Animal Physiology,University of the Basque Country (UPV-EHU), Biocruces Health Research Institute, 2Endocrinology and Diabetes Research Group, Biocruces Health Research Institute,UPV-EHU, CIBERDEM

Capitoli

  • 00:01Titolo
  • 01:29Protocol
  • 02:10Biopsy homogenization
  • 03:25Cytoplasm isolation
  • 04:21Nuclear isolation
  • 07:29Representative Results
  • 09:27Conclusions

Summary

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Here, we present a protocol to perform a simple cellular fractionation for the subcellular separation of cytoplasmic and nuclear proteins in human fresh and frozen intestinal biopsies.

Tags

Keywords: Subcellular FractionationGastrointestinal SpecimensProtein LocalizationIntestinal BiopsyNuclear And Cytoplasmic FractionsHomogenizationDetergent LysisCentrifugation

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