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毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析
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Biochimica
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JoVE Journal Biochimica
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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毛细管电泳与亲和毛细管电泳对金属离子 AtHIRD11 固有紊乱与结合的分析

DOI:
10.3791/57749-v

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07:54 min

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August 22, 2018

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, , ,
1Institute of Medicinal and Pharmaceutical Chemistry,TU Braunschweig

Capitoli

  • 00:04Titolo
  • 00:34Preparation of Capillaries
  • 01:45Vial Preparation and Affinity Capillary Electrophoretic Separation
  • 03:34Affinity Capillary Electrophoretic Analysis – Preparation for ACE Methods
  • 05:20Results: Characterization of AtHIRD11 by Affinity Capillary Electrophoresis and Capillary Gel Electrophoresis
  • 06:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议结合毛细管电泳对蛋白质样品的特性进行了表征, 并对带电配体进行了快速结合筛选。推荐用于具有弹性结构的蛋白质, 如内在紊乱的蛋白质, 以确定不同构象的结合的差异。

Tags

Intrinsic DisorderMetal Ion BindingCapillary Gel ElectrophoresisAffinity Capillary ElectrophoresisConformational ChangesGlass CapillaryPolyamide CoatingDetection WindowCalcium ChlorideMetal Salt SolutionsTris BufferAcetanilide EOF MarkerSample SolutionHydrodynamic InjectionElectrophoretic Separation

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