Rivista
/
Biochimica
/
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
JoVE Journal
Biochimica
This content is Free Access.
JoVE Journal Biochimica
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques

DOI:
10.3791/57750-v

•

08:58 min

•

July 05, 2018

•

, , , ,
1Program in Molecular Medicine,The Hospital for Sick Children Research Institute, 2Department of Biochemistry,University of Toronto, 3Department of Immunology,University of Toronto

Capitoli

  • 00:05Titolo
  • 00:38Purification of Soluble Glycoprotein from HEK293 Supernatant
  • 02:08Crystallization of Glycoproteins
  • 03:44Soaking Glycoprotein Crystals with its Ligand
  • 04:33Characterization of Fab and Small Molecule Binding to the Glycoprotein
  • 07:34Results: Characterization of Glycoproteins
  • 08:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

We present approaches for the biophysical and structural characterization of glycoproteins with the immunoglobulin fold by biolayer interferometry, isothermal titration calorimetry, and X-ray crystallography.

Tags

Keywords: GlycoproteinsImmunoglobulin FoldX-ray CrystallographyBiophysical TechniquesProtein FunctionN-linked GlycansAntibioticsStructural CharacterizationProtein PurificationSize Exclusion ChromatographyDeglycosylationProtein ConcentrationCrystallization

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/57750?article_type=v

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
check_url/it/57750?article_type=v

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
check_url/it/57750?article_type=v

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
check_url/it/57750?article_type=v

Characterization of Proteins by Size-Exclusion Chromatography Coupled to Multi-Angle Light Scattering (SEC-MALS)
check_url/it/57750?article_type=v

An All-in-one Sample Holder for Macromolecular X-ray Crystallography with Minimal Background Scattering
check_url/it/57750?article_type=v

Cell Culture on Silicon Nitride Membranes and Cryopreparation for Synchrotron X-ray Fluorescence Nano-analysis
check_url/it/57750?article_type=v

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence
check_url/it/57750?article_type=v

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050
check_url/it/57750?article_type=v

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
check_url/it/57750?article_type=v

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Read Article