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Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
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Capitoli
- 00:04Titolo
- 00:46Amoebae: M. Abscessus Tn Mutant Co-culture
- 03:32Intracellular Mycobacteria RNA Extraction for RNAseq Analysis
- 07:18Results: Mycobacterial mRNA Extraction Analysis
- 07:47Conclusion
Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.
