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猕猴免疫缺陷病毒感染 CD4+ T 细胞单细胞定量的 mRNA 和表面蛋白的表达
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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猕猴免疫缺陷病毒感染 CD4+ T 细胞单细胞定量的 mRNA 和表面蛋白的表达

DOI:
10.3791/57776-v

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13:13 min

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September 25, 2018

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, , , ,
1US Military HIV Research Program, Henry M. Jackson Foundation,Walter Reed Army Institute of Research, 2Vaccine Research Center, NIAID,NIH

Capitoli

  • 00:04Titolo
  • 00:46Gene Expression Assay Preparation and Surface Staining of Viable Cells
  • 03:18Prepare Cell Collection Plates, Perform FACS Sort, and Generate cDNA
  • 05:17Multiplex qPCR on the Biomark Platform
  • 11:26Results: Single-cell Quantitation of mRNA and Surface Protein Expression
  • 12:56Conclusion

Summary

Traduzione automatica

Traduzione automatica

描述的方法, 以定量的表达96基因和18表面蛋白的单细胞前体, 允许鉴定差异表达基因和蛋白质的病毒感染细胞相对未感染的细胞。我们采用这种方法来研究与恒河猴分离的 SIV 感染的 CD4+ T 细胞。

Tags

Single-cell QuantitationMRNASurface ProteinSimian Immunodeficiency VirusCD4+ T CellsRhesus MacaquesHost-pathogen InteractionsCell SortingFlow CytometryGene Expression AssaysSurface StainingCell SuspensionCell ViabilityAntibody CocktailCell Strainer

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