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Enkelt-celle analyse af Immunophenotype og cytokin produktion i perifere blod via masse flowcytometri
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

Enkelt-celle analyse af Immunophenotype og cytokin produktion i perifere blod via masse flowcytometri

DOI:
10.3791/57780-v

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12:36 min

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June 26, 2018

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, , , ,
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2OMNI Biomarkers, Development Sciences,Genentech, 3Department of Pediatrics, Division of Allergy and Immunology,University of Colorado School of Medicine

Capitoli

  • 00:04Titolo
  • 01:10Stimulation and Processing of Peripheral Whole Blood
  • 05:27Barcode of Lysed/Fixed Blood Cells
  • 09:44Results: Experimental Workflow and Representative Gating Strategy for the Identification of CD14hi Monocytes
  • 11:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskriver vi en enkelt celle proteom tilgang til at vurdere immun fænotypiske og funktionelle (intracellulære cytokin induktion) ændringer i det perifere blodprøver, analyseret via masse flowcytometri.

Tags

Single-cell AnalysisImmunophenotypeCytokine ProductionPeripheral Whole BloodMass CytometryAutoimmunityCell FrequencyCytokine ProductionSystems ImmunologyAutoimmune DiseaseSLEWhole Blood ProcessingRuxolitinibLyse/fix BufferCell RecoveryAntibody Staining

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