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Induction and Validation of Cellular Senescence in Primary Human Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Induction and Validation of Cellular Senescence in Primary Human Cells

Induction and Validation of Cellular Senescence in Primary Human Cells

DOI:
10.3791/57782-v

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08:18 min

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June 20, 2018

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, ,
1European Research Institute for the Biology of Aging,University of Groningen, University Medical Center Groningen

Capitoli

  • 00:04Titolo
  • 00:43Doxorubicin-induced Senescence
  • 01:54Senescence-associated ß-galactosidase Staining
  • 03:35EdU Incorporation Assay
  • 06:30Results: Quantification of Senescence-associated Markers
  • 07:17Conclusion

Summary

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Here, we discuss a series of protocols for induction and validation of cellular senescence in cultured cells. We focus on different senescence-inducing stimuli and describe the quantification of common senescence-associated markers. We provide technical details using fibroblasts as a model, but the protocols can be adapted to various cellular models.

Tags

Cellular SenescencePrimary Human CellsInductionValidationBeta-galactosidase StainingDoxorubicinFibroblastsAgingCancerCell CultureMicroscopy

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