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原发性细胞衰老的诱导与鉴定
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Induction and Validation of Cellular Senescence in Primary Human Cells
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原发性细胞衰老的诱导与鉴定

DOI:
10.3791/57782-v

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08:18 min

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June 20, 2018

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, ,
1European Research Institute for the Biology of Aging,University of Groningen, University Medical Center Groningen

Capitoli

  • 00:04Titolo
  • 00:43Doxorubicin-induced Senescence
  • 01:54Senescence-associated ß-galactosidase Staining
  • 03:35EdU Incorporation Assay
  • 06:30Results: Quantification of Senescence-associated Markers
  • 07:17Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们讨论了一系列的诱导和确认细胞衰老在培养细胞的协议。我们专注于不同的衰老诱发刺激, 并描述了常见的衰老相关标记的量化。我们提供的技术细节, 以成纤维细胞作为模型, 但协议可以适应各种细胞模型。

Tags

Cellular SenescencePrimary Human CellsInductionValidationBeta-galactosidase StainingDoxorubicinFibroblastsAgingCancerCell CultureMicroscopy

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