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Bioengineering

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JoVE Journal Bioengineering
Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope
 

Stiffness Measurement of Soft Silicone Substrates for Mechanobiology Studies Using a Widefield Fluorescence Microscope

Article DOI: 10.3791/57797-v • 07:02 min • July 3rd, 2018
July 3rd, 2018 •
*1, *1, *2, *1, 1, 1
1Department of Mechanical & Aerospace Engineering, Old Dominion University, 2Department of Biological Sciences, Old Dominion University
* These authors contributed equally

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Substrates with stiffness in the kilopascal-range are useful to study the response of cells to physiologically relevant micro-environment stiffness. Using just a widefield fluorescence microscope, the Young's modulus of soft silicone gels can be determined using an indentation with a suitable sphere.

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Stiffness Measurement Soft Silicone Substrates Mechanobiology Studies Widefield Fluorescence Microscope Young's Modulus Silicon Polyacrylamide Gel Soft Isotropic Linear Elastic Material Self-stretching Experiments Silicone Elastomer Kit Petri Dish Vacuum Chamber Hot Plate
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