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Efficient Purification and LC-MS/MS-based Assay Development for Ten-Eleven Translocation-2 5-Methylcytosine Dioxygenase
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Capitoli
- 00:04Titolo
- 00:45Cloning and Purification of Untagged Human TET2 Dioxygenase
- 05:305mC Oxidation by TET2 Dioxygenase
- 07:02Quantitative Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)-based Assay Development
- 08:31Results: Investigating the Role of the TET2 Enzyme in Normal Development and Disease
- 09:26Conclusion
Here, we present a protocol for an efficient single step purification of the active untagged human ten-eleven translocation-2 (TET2) 5-methylcytosine dioxygenase using ion-exchange chromatography and its assay using a liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based approach.
