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High-Resolution Comparison of Bacterial Conjugation Frequencies
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JoVE Journal Genetica
High-Resolution Comparison of Bacterial Conjugation Frequencies

High-Resolution Comparison of Bacterial Conjugation Frequencies

DOI:
10.3791/57812-v

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05:18 min

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January 10, 2019

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, ,
1Applied Chemistry and Biochemical Engineering Course, Department of Engineering, Graduate School of Integrated Science and Technology,Shizuoka University, 2Department of Bioscience, Graduated School of Science and Technology,Shizuoka University, 3Japan Collection of Microorganisms,RIKEN BioResource Research Center

Capitoli

  • 00:04Titolo
  • 00:28Most Probable Number (MPN) Conjugation Frequency Calculation
  • 02:37Results: Representative Probability of Donor-Initiated Conjugation Between Plasmids
  • 05:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

With an aim to understand the behaviors of various bacterial conjugative DNA elements under different conditions, we describe a protocol for detecting differences in conjugation frequency, with high resolution, to estimate how efficiently the donor bacterium initiates conjugation.

Tags

Bacterial ConjugationPlasmid DNA TransferMost Probable NumberConjugation FrequencyHigh-resolution ComparisonMicrobiologyPlasmidTransconjugants

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